Zeuterin/EsterilSol™:
Injectable Sterilant for Male Dogs


Zeuterin™ (U.S.) and EsterilSol™ (same product, non-U.S. brand name) is a product developed to chemically sterilize male dogs through a process called "zinc neutering." The formulation was initially introduced in the U.S. under the name Neutersol and is the first permanent non-surgical sterilant to receive regulatory approval from the U.S. Food and Drug Administration (FDA). The formulation is now being marketed under the names Zeuterin and EsterilSol by Ark Sciences, LLC. Zeuterin/EsterilSol is the chemical compound zinc gluconate neutralized by arginine. It provides permanent sterility in a single treatment and makes it possible to sterilize male dogs who might otherwise contribute to homeless pet populations. Dogs treated with Zeuterin/EsterilSol retain their testicles (though testicles do change in size and texture). For many animal shelters and sterilization clinics, this approach will require less time, money, and infrastructure than surgical castration, helping these organizations reach further with limited resources.

This formulation currently has regulatory approval in the U.S., Mexico, Colombia, Bolivia, and Panama. Ark Sciences launched Zeuterin commercially on February 17, 2014; you can download the company’s press release here.

Ark Sciences reports plans to seek regulatory approval in additional countries moving forward. Currently, this product may be able to be used with special permission on a limited basis in some countries in which it does not have regulatory approval.

ACC&D’s goal is to help organizations explore how EsterilSol/Zeuterin may help them sterilize more dogs and/or conserve their limited resources, and, where it is a good fit, to help organizations utilize it effectively. Please use the links below to learn more. The Product Profile and Position Paper provides a thorough overview and is a great place to start. You are also welcome to contact us with questions at info@acc-d.org.

Technical Information and other Publications
Freedom of Information Summary (results of studies submitted to the FDA for regulatory review)
Injectable sterilant for dogs returning to market
-Edie Lau, VIN News Service
Comparison of intratesticular injection of zinc gluconate versus surgical castration to sterilize male dogs
-Levy et al., published in the American Journal of Veterinary Research
Neutersol: A Clinical Perspective (abstract of presentation at the 2009 North American Veterinary Conference)
- James Weedon, DVM, MPH, DACVPM

Information from Ark Sciences
Ark Sciences website for Zeuterin/EsterilSol

Proceedings from related sessions at ACC&D's 5th International Symposium (2013)
(Click here for complete symposium proceedings and individual presentation abstracts/handouts)
Zeuterin in male dogs and cats (narrated PowerPoint) - Dr. Byron Maas
Panel of ZeuterinTM/EsterilsolTM users from the U.S. and abroad (video) - Dr. Mary Blankevoort (Portland Animal Welfare Team), Dr. Kelly Farrell (First Coast No More Homeless Pets), Dr. Ismail Thoya (Kenya Society for the Protection & Care of Animals), and Dr. Amber Valinski (The Shot Spot)
Behaviour assessment of male free-roaming dogs following surgical and non-surgical sterilization in Puerto Natales, Chile - Dr. Raphael Vanderstichel
The effect of surgical and chemical sterilization on canine testosterone levels - Dr. Raphael Vanderstichel
Effect of intratesticular injection of zinc gluconate neutralized by arginine on reproductive parameters - Dr. Min Wang


Proceedings from related sessions at ACC&D’s 4th International Symposium (2010)
(Click here for full symposium proceedings)
Chemical Sterilization: Understanding Opportunities and Challenges- Emma Clifford (PowerPoint presentation)
Chemical Sterilization: Understanding Opportunities and Challenges- Dr. Julie Levy (PowerPoint presentation)
Chemical Sterilization: Understanding Opportunities and Challenges- Dr. Enid Stiles (PowerPoint presentation)
Chemical Sterilization: Understanding Opportunities and Challenges- Panel Discussion (video)

Proceedings from related sessions at ACC&D’s 3rd International Symposium (2006)
(Click here for full symposium proceedings)
Session Overview – Dr. Julie Levy
Case Study: Outreach in the U.S. and Mexico, The Face of Success – Sean Hawkins
Report on Field Use in 10,000 Dogs in Mexico – Dr. Carlos Esquivel