Calcium chloride is being researched as an intratesticular injectable sterilant for male dogs and cats. While research on this chemical sterilant goes back to the 1970s, a majority of peer-reviewed and published data emerged in the past decade. Recent research can be credited largely to support from the Parsemus Foundation, which advocates for calcium chloride as an option that can treat both cats and dogs, is inexpensive, is readily available, and may reduce testosterone to lower levels than the injectable sterilant Zeuterin™ for male dogs.
Controlled and field studies have taken place in the U.S., Italy, and India to gather more information on calcium chloride as a non-surgical sterilization option for male dogs and cats. The calcium chloride-based compound used for sterilization is not presently approved by any regulatory agency, and it is created through compounding ingredients. ACC&D believes that the current use of calcium chloride as an injectable sterilant should be considered experimental.
In October 2014, two peer-reviewed research papers on calcium chloride were published in the open-access journal Acta Veterinaria Scandinavica. "Alcohol diluent provides the optimal formulation for calcium chloride non-surgical sterilization in dogs" can be accessed here; "A dose-finding, long-term study on the use of calcium chloride in saline solution as a method of nonsurgical sterilization in dogs" can be accessed here. Comprehensive reviews and information on calcium chloride can be accessed below.
Learn more about calcium chloride studies for male cats and dogs with these resources:
ACC&D statement and position on calcium chloride
ACC&D summary of research on calcium chloride
Contraception & Fertility Control in Dogs & Cats (ACC&D e-book)
5th International Symposium (2013) presentations
Calcium Chloride in male dogs and cats * Elaine Lissner
PowerPoint Slides Abstract Presentation with Audio
Could your organization use Calcium Chloride? Nuts and Bolts * Elaine Lissner
PowerPoint Slides Abstract Presentation with Video (begins at 37:53)
Clinical evaluation of efficacy and safety of Calcium Chloride associated with ethyl alcohol and lidocaine for non-surgical sterilization of sexually mature canine and feline adults * Dr. Kuladip Jana
PowerPoint Slides Abstract Presentation with Audio Session Q&A with Audio
Intratesticular injection of a calcium chloride tincture in the dog * Dr. Raffaella Leoci
4th International Symposium (2010) presentations
History of Calcium Chloride injectable sterilization in male dogs and first report of use in cats * Dr. Kuladip Jana
Pilot Study: Intratesticular Injection Induces Sterility in Male Cats * Dr. Alper Baran