New Possibilities With
Non-surgical Solutions

Notice: Zeuterin was commercially launched in the U.S. in 2014, following approval and use in several other countries by the name EsterilSol. The product, which was manufactured by Ark Sciences, is not currently being produced or distributed in any country due to the manufacturer’s financial challenges. While there is presently no indication of returning to the market, the formulation retains approval by the U.S. Food & Drug Administration (FDA).


Zeuterin™ (also labeled as EsterilSol™ in some countries) sterilizes male dogs by intratesticular injection. Composed of zinc gluconate neutralized by arginine, Zeuterin offers sterilization in one treatment, without general anesthesia, and without removing testicles. Launched in the U.S. in February 2014 by Ark Sciences, LLC, Zeuterin is presently the only non-surgical sterilant with U.S. Food & Drug Administration (FDA) approval.

Prior to U.S. launch, ACC&D sought to learn more about Zeuterin/EsterilSol and its potential to add value to sterilization efforts. We supported projects to explore its reception by communities, study its potential impact on canine behavior, and share innovative strategies to incorporate Zeuterin into animal shelters/rescues and veterinary practices in test market. The common thread among all these initiatives is ACC&D’s commitment to generate information about this new technology and help communities and individuals determine how it could best provide an alternative to castration, improve animal welfare and humanely reduce numbers of unwanted dogs.

Read on to learn about the product materials and information available on our website.

Product Profile and Position Paper: ACC&D's 4P offers an objective and independent overview of Zeuterin, including its mechanism of action; use and effectiveness; health, safety, and behavioral considerations; and more. A recommended first stop for information on this product!  

ACC&D Symposia presentations: Our 3rd, 4th, 5th and 6th International Symposia included multiple presentations, panels, and posters on Zeuterin and its predecessor, Neutersol. Here you can access all symposia resources on this topic for free.

"Neutersol" FOIA Summary: A resource available through the Freedom of Information Act summarizing product safety characteristics submitted to the US Food & Drug Administration (FDA) for approval. (Zeuterin has the same chemical composition as Neutersol but requires training and certification prior to purchase and use.)

"Prizes for Pioneers" Zeuterin Contest: In conjunction with Zeuterin’s launch in the U.S., in 2014 we held a contest to encourage organizations and veterinarians using Zeuterin to share their best and most creative strategies for effectively incorporating the product to sterilize more animals, use resources more effectively, and reach male dogs who otherwise would not be neutered. Here you can view profiles of these organizations and learn about the strategies that they've implemented.    

Reports from the field: When Zeuterin was available, several veterinarians, animal welfare organizations, and shelters incorporated the product  as a complement to surgical castration. Here you can read about organizations that embraced particularly interesting and innovative ways of using Zeuterin to treat more animals and save more lives. Though Zeuterin is not currently available, the ideas have application to other non-surgical approaches.

Zeuterin, Castration, and Canine Behavior: How does Zeuterin influence behavior? What is the impact of castration on behavior? And how do both compare with intact animals? The reality is that we have very little objective information on these topics. ACC&D supported an initiative to gather data on free-roaming dog populations, the results of which contribute to better understanding how different procedures can influence behavior.

EsterilSol Small Grants Program: Between 2009 and 2010, ACC&D offered small grants to five South American and African organizations. Funds supported work to increase sterilization capacity and improve understandings of field use of EsterilSol. Although the product is not available at this time, ideas and learnings have application to other non-surgical approaches.

EsterilSol Guatemala field study: Todos Santos, Guatemala faces numerous obstacles to surgical castration: limited resources, primitive field conditions, and a cultural aversion to castration. ACC&D supported an innovative project to explore how EsterilSol might aid population control and animal welfare efforts in this community—and generate findings to help other communities facing similar challenges. Learnings are relevant for other non-surgical fertility control options, as well.  

Available Products


News & Updates